Development of an experimental model for radiation-induced inhibition of cranial bone regeneration
정홍문, 이정은, 이승준, 이정태, 권태엽, 권태건,
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정홍문 ( Jung Hong-Moon ) - Kyungpook National University School of Dentistry Department of Oral and Maxillofacial Surgery
이정은 ( Lee Jeong-Eun ) - Kyungpook National University School of Medicine Department of Radiation Oncology
이승준 ( Lee Seoung-Jun ) - Kyungpook National University Hospital Department of Radiation Oncology
이정태 ( Lee Jung-Tae ) - Kyungpook National University School of Dentistry Department of Oral and Maxillofacial Surgery
권태엽 ( Kwon Tae-Yub ) - Kyungpook National University School of Dentistry Department of Dental Biomaterials
권태건 ( Kwon Tae-Geon ) - Kyungpook National University School of Dentistry Department of Oral and Maxillofacial Surgery
Abstract
Background: Radiation therapy is widely employed in the treatment of head and neck cancer. Adverse effects of therapeutic irradiation include delayed bone healing after dental extraction or impaired bone regeneration at the irradiated bony defect. Development of a reliable experimental model may be beneficial to study tissue regeneration in the irradiated field. The current study aimed to develop a relevant animal model of post-radiation cranial bone defect.
Methods: A lead shielding block was designed for selective external irradiation of the mouse calvaria. Critical-size calvarial defect was created 2 weeks after the irradiation. The defect was filled with a collagen scaffold, with or without incorporation of bone morphogenetic protein 2 (BMP-2) (1 μg/ml). The non-irradiated mice treated with or without BMP-2-included scaffold served as control. Four weeks after the surgery, the specimens were harvested and the degree of bone formation was evaluated by histological and radiographical examinations.
Results: BMP-2-treated scaffold yielded significant bone regeneration in the mice calvarial defects. However, a single fraction of external irradiation was observed to eliminate the bone regeneration capacity of the BMP-2-incorporated scaffold without influencing the survival of the animals.
Conclusion: The current study established an efficient model for post-radiation cranial bone regeneration and can be applied for evaluating the robust bone formation system using various chemokines or agents in unfavorable, demanding radiation-related bone defect models.
키워드
Radiation; Bone; Regeneration; Calvaria; Defect
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